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enzyme linked immunosorbent assay kit  (Cusabio)


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    Cusabio enzyme linked immunosorbent assay kit
    Enzyme Linked Immunosorbent Assay Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 93/100, based on 27 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/immunosorbent+assay+kit/Mouse+Corticosterone%2CCORT+ELISA+Kit/pmc13664201-92-11-16
    Average 93 stars, based on 27 article reviews
    enzyme linked immunosorbent assay kit - by Bioz Stars, 2026-10
    93/100 stars

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    Article Title: Serum insulin-like growth factor-1 and leptin concentrations in Black Bengal goats at different ages and gestation periods and their relationship to kid growth performance
    Article Snippet: Leptin was measured using a goat-specific leptin commercial sandwich enzyme-linked immunosorbent assay kit (CUSABIO, catalog number: CSB-E12834G, Biotech Co., Ltd, China) according to the manufacturer’s instructions.

    Article Title: Targeting bone marrow mesenchymal stromal cell–derived IL-6 to overcome acute myeloid leukemia chemoresistance
    Article Snippet: The clarified 80 supernatants were then used to quantify IL-6 concentrations using a human IL-6 enzyme-linked 81 immunosorbent assay kit (Cusabio).

    Article Title: Potential clinical value of fibrinogen-like protein 1 as a serum biomarker for the identification of diabetic cardiomyopathy.
    Article Snippet: Erum FGL-1 levels were determined using a commercial enzyme-linked immunosorbent assay kit provided by Cusabio (CSB-EL008653HU, Houston, TX, USA)32.

    Article Title: Potential clinical value of fibrinogen-like protein 1 as a serum biomarker for the identification of diabetic cardiomyopathy
    Article Snippet: Erum FGL-1 levels were determined using a commercial enzyme-linked immunosorbent assay kit provided by Cusabio (CSB-EL008653HU, Houston, TX, USA) .

    Article Title: Exosomes Derived from Hypertrophic Scar Fibroblasts Suppress Melanogenesis in Normal Human Epidermal Melanocytes
    Article Snippet: Melanin content in the samples was measured using a human melanin enzyme-linked immunosorbent assay kit (CSB-E14051h, Cusabio Technology, Houston, TX, USA) according to the manufacturer’s instructions.

    Article Title: Exosomes Derived from Hypertrophic Scar Fibroblasts Suppress Melanogenesis in Normal Human Epidermal Melanocytes.
    Article Snippet: Melanin content in the samples was measured using a human melanin enzyme-linked immunosorbent assay kit (CSB-E14051h, Cusabio Technology, Houston, TX, USA) according to the manufacturer’s instructions.

    Enzyme-linked Immunosorbent Assay:

    Article Title: Correlation Between Growth Differentiation Factor-15 and Peripheral Neuropathy in Patients with Type 2 Diabetes Mellitus
    Article Snippet: .. An enzyme-linked immunosorbent assay kit (Cusabio Biotechnology, China) was used to measure GDF-15 (pg/mL). ..

    Article Title: Neutrophil elastase promotes low molecular weight cyclin E1 formation to accelerate osteosarcoma proliferation
    Article Snippet: .. Enzyme-linked immunosorbent assay (ELISA) was performed according to the manufacturer’s instructions, using an enzyme‐linked immunosorbent assay kit (CSB-EL007587HU, CUSABIO, China) for ELA2 and an ELISA assay kit (RX102517H, Rruixin Bio, China) for NETs. .. The cell cycle assay was performed using PI staining, and the cell cycle and apoptosis analysis kit (C1052, Beyotime, China) was used according to the operator’s manual.



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    AA exacerbated podocyte injury in PAN‐induced rats. Eight‐week‐old male Sprague–Dawley rats (180–200 g) were subjected to puromycin aminonucleoside (PAN)‐induced podocyte injury by a single intraperitoneal injection of PAN (7 mg/100 g body weight). Ten days after PAN injection, rats in the PAN+AA group received arachidonic acid (AA, 3 mg/kg, i.p.) to evaluate AA‐associated exacerbation of established PAN‐induced injury. (A) Serum AA levels were measured by <t>ELISA.</t> Serum albumin, serum creatinine, 24‐h urinary albumin, serum cholesterol and serum triglyceride levels were measured as indicators of renal function and metabolic status ( n = 6). (B) Representative haematoxylin and eosin (H&E), Masson's trichrome, and periodic acid‐Schiff (PAS) staining of kidney sections ( n = 6). Scale bar, 20 μm. (C) Representative transmission electron microscopy images of podocyte foot processes and quantitative analysis of glomerular basement membrane (GBM) thickness, foot‐process width, and the number of foot processes per μm GBM ( n = 3). Scale bar, 5.0 μm. (D) Representative immunofluorescence staining of nephrin in glomeruli. Nephrin is shown in red and nuclei are counterstained with DAPI in blue ( n = 6). Scale bar, 20 μm. (E) Representative Western blot analysis and quantification of nephrin protein expression in renal tissue lysates ( n = 6). Data are expressed as the mean ± SD and analysed by one‐way ANOVA with Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. AA, arachidonic acid; GBM, glomerular basement membrane; i.p., intraperitoneal; MCD, minimal change disease; PAN, puromycin aminonucleoside.
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    enzyme linked immunosorbent assay kit  (Cusabio)
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    AA exacerbated podocyte injury in PAN‐induced rats. Eight‐week‐old male Sprague–Dawley rats (180–200 g) were subjected to puromycin aminonucleoside (PAN)‐induced podocyte injury by a single intraperitoneal injection of PAN (7 mg/100 g body weight). Ten days after PAN injection, rats in the PAN+AA group received arachidonic acid (AA, 3 mg/kg, i.p.) to evaluate AA‐associated exacerbation of established PAN‐induced injury. (A) Serum AA levels were measured by <t>ELISA.</t> Serum albumin, serum creatinine, 24‐h urinary albumin, serum cholesterol and serum triglyceride levels were measured as indicators of renal function and metabolic status ( n = 6). (B) Representative haematoxylin and eosin (H&E), Masson's trichrome, and periodic acid‐Schiff (PAS) staining of kidney sections ( n = 6). Scale bar, 20 μm. (C) Representative transmission electron microscopy images of podocyte foot processes and quantitative analysis of glomerular basement membrane (GBM) thickness, foot‐process width, and the number of foot processes per μm GBM ( n = 3). Scale bar, 5.0 μm. (D) Representative immunofluorescence staining of nephrin in glomeruli. Nephrin is shown in red and nuclei are counterstained with DAPI in blue ( n = 6). Scale bar, 20 μm. (E) Representative Western blot analysis and quantification of nephrin protein expression in renal tissue lysates ( n = 6). Data are expressed as the mean ± SD and analysed by one‐way ANOVA with Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. AA, arachidonic acid; GBM, glomerular basement membrane; i.p., intraperitoneal; MCD, minimal change disease; PAN, puromycin aminonucleoside.
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    AA exacerbated podocyte injury in PAN‐induced rats. Eight‐week‐old male Sprague–Dawley rats (180–200 g) were subjected to puromycin aminonucleoside (PAN)‐induced podocyte injury by a single intraperitoneal injection of PAN (7 mg/100 g body weight). Ten days after PAN injection, rats in the PAN+AA group received arachidonic acid (AA, 3 mg/kg, i.p.) to evaluate AA‐associated exacerbation of established PAN‐induced injury. (A) Serum AA levels were measured by <t>ELISA.</t> Serum albumin, serum creatinine, 24‐h urinary albumin, serum cholesterol and serum triglyceride levels were measured as indicators of renal function and metabolic status ( n = 6). (B) Representative haematoxylin and eosin (H&E), Masson's trichrome, and periodic acid‐Schiff (PAS) staining of kidney sections ( n = 6). Scale bar, 20 μm. (C) Representative transmission electron microscopy images of podocyte foot processes and quantitative analysis of glomerular basement membrane (GBM) thickness, foot‐process width, and the number of foot processes per μm GBM ( n = 3). Scale bar, 5.0 μm. (D) Representative immunofluorescence staining of nephrin in glomeruli. Nephrin is shown in red and nuclei are counterstained with DAPI in blue ( n = 6). Scale bar, 20 μm. (E) Representative Western blot analysis and quantification of nephrin protein expression in renal tissue lysates ( n = 6). Data are expressed as the mean ± SD and analysed by one‐way ANOVA with Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. AA, arachidonic acid; GBM, glomerular basement membrane; i.p., intraperitoneal; MCD, minimal change disease; PAN, puromycin aminonucleoside.
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    AA exacerbated podocyte injury in PAN‐induced rats. Eight‐week‐old male Sprague–Dawley rats (180–200 g) were subjected to puromycin aminonucleoside (PAN)‐induced podocyte injury by a single intraperitoneal injection of PAN (7 mg/100 g body weight). Ten days after PAN injection, rats in the PAN+AA group received arachidonic acid (AA, 3 mg/kg, i.p.) to evaluate AA‐associated exacerbation of established PAN‐induced injury. (A) Serum AA levels were measured by <t>ELISA.</t> Serum albumin, serum creatinine, 24‐h urinary albumin, serum cholesterol and serum triglyceride levels were measured as indicators of renal function and metabolic status ( n = 6). (B) Representative haematoxylin and eosin (H&E), Masson's trichrome, and periodic acid‐Schiff (PAS) staining of kidney sections ( n = 6). Scale bar, 20 μm. (C) Representative transmission electron microscopy images of podocyte foot processes and quantitative analysis of glomerular basement membrane (GBM) thickness, foot‐process width, and the number of foot processes per μm GBM ( n = 3). Scale bar, 5.0 μm. (D) Representative immunofluorescence staining of nephrin in glomeruli. Nephrin is shown in red and nuclei are counterstained with DAPI in blue ( n = 6). Scale bar, 20 μm. (E) Representative Western blot analysis and quantification of nephrin protein expression in renal tissue lysates ( n = 6). Data are expressed as the mean ± SD and analysed by one‐way ANOVA with Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. AA, arachidonic acid; GBM, glomerular basement membrane; i.p., intraperitoneal; MCD, minimal change disease; PAN, puromycin aminonucleoside.
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    Skin-tissue <t> IL-22 </t> concentrations across the experimental groups.
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    Skin-tissue <t> IL-22 </t> concentrations across the experimental groups.
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    Image Search Results


    AA exacerbated podocyte injury in PAN‐induced rats. Eight‐week‐old male Sprague–Dawley rats (180–200 g) were subjected to puromycin aminonucleoside (PAN)‐induced podocyte injury by a single intraperitoneal injection of PAN (7 mg/100 g body weight). Ten days after PAN injection, rats in the PAN+AA group received arachidonic acid (AA, 3 mg/kg, i.p.) to evaluate AA‐associated exacerbation of established PAN‐induced injury. (A) Serum AA levels were measured by ELISA. Serum albumin, serum creatinine, 24‐h urinary albumin, serum cholesterol and serum triglyceride levels were measured as indicators of renal function and metabolic status ( n = 6). (B) Representative haematoxylin and eosin (H&E), Masson's trichrome, and periodic acid‐Schiff (PAS) staining of kidney sections ( n = 6). Scale bar, 20 μm. (C) Representative transmission electron microscopy images of podocyte foot processes and quantitative analysis of glomerular basement membrane (GBM) thickness, foot‐process width, and the number of foot processes per μm GBM ( n = 3). Scale bar, 5.0 μm. (D) Representative immunofluorescence staining of nephrin in glomeruli. Nephrin is shown in red and nuclei are counterstained with DAPI in blue ( n = 6). Scale bar, 20 μm. (E) Representative Western blot analysis and quantification of nephrin protein expression in renal tissue lysates ( n = 6). Data are expressed as the mean ± SD and analysed by one‐way ANOVA with Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. AA, arachidonic acid; GBM, glomerular basement membrane; i.p., intraperitoneal; MCD, minimal change disease; PAN, puromycin aminonucleoside.

    Journal: Journal of Cellular and Molecular Medicine

    Article Title: Arachidonic Acid Disrupts PKM2 ‐Linked Glycolysis‐Related Metabolism and Exacerbates PAN ‐Induced Podocyte Injury

    doi: 10.1111/jcmm.71404

    Figure Lengend Snippet: AA exacerbated podocyte injury in PAN‐induced rats. Eight‐week‐old male Sprague–Dawley rats (180–200 g) were subjected to puromycin aminonucleoside (PAN)‐induced podocyte injury by a single intraperitoneal injection of PAN (7 mg/100 g body weight). Ten days after PAN injection, rats in the PAN+AA group received arachidonic acid (AA, 3 mg/kg, i.p.) to evaluate AA‐associated exacerbation of established PAN‐induced injury. (A) Serum AA levels were measured by ELISA. Serum albumin, serum creatinine, 24‐h urinary albumin, serum cholesterol and serum triglyceride levels were measured as indicators of renal function and metabolic status ( n = 6). (B) Representative haematoxylin and eosin (H&E), Masson's trichrome, and periodic acid‐Schiff (PAS) staining of kidney sections ( n = 6). Scale bar, 20 μm. (C) Representative transmission electron microscopy images of podocyte foot processes and quantitative analysis of glomerular basement membrane (GBM) thickness, foot‐process width, and the number of foot processes per μm GBM ( n = 3). Scale bar, 5.0 μm. (D) Representative immunofluorescence staining of nephrin in glomeruli. Nephrin is shown in red and nuclei are counterstained with DAPI in blue ( n = 6). Scale bar, 20 μm. (E) Representative Western blot analysis and quantification of nephrin protein expression in renal tissue lysates ( n = 6). Data are expressed as the mean ± SD and analysed by one‐way ANOVA with Bonferroni correction. * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001; ns, not significant. AA, arachidonic acid; GBM, glomerular basement membrane; i.p., intraperitoneal; MCD, minimal change disease; PAN, puromycin aminonucleoside.

    Article Snippet: Serum arachidonic acid (AA) levels were measured using a commercially available enzyme‐linked immunosorbent assay (ELISA) kit (Elabscience, catalogue no. E‐EL‐0051) according to the manufacturer's instructions.

    Techniques: Injection, Enzyme-linked Immunosorbent Assay, Staining, Transmission Assay, Electron Microscopy, Analysis, Membrane, Immunofluorescence, Western Blot, Expressing, Tissue

    Skin-tissue IL-22 concentrations across the experimental groups.

    Journal: Frontiers in Medicine

    Article Title: Effects of topical Averrhoa bilimbi leaf extract cream on IL-22 and filaggrin in a murine model of atopic dermatitis

    doi: 10.3389/fmed.2026.1883375

    Figure Lengend Snippet: Skin-tissue IL-22 concentrations across the experimental groups.

    Article Snippet: Cutaneous IL-22 concentrations were quantified using a mouse IL-22 enzyme-linked immunosorbent assay kit (Elabscience, catalog no. E-EL-M2446, Wuhan, China) according to the manufacturer's instructions.

    Techniques: Control, Positive Control

    Skin-tissue IL-22 concentrations after topical administration of A. bilimbi leaf extract cream in a mouse model of atopic dermatitis.

    Journal: Frontiers in Medicine

    Article Title: Effects of topical Averrhoa bilimbi leaf extract cream on IL-22 and filaggrin in a murine model of atopic dermatitis

    doi: 10.3389/fmed.2026.1883375

    Figure Lengend Snippet: Skin-tissue IL-22 concentrations after topical administration of A. bilimbi leaf extract cream in a mouse model of atopic dermatitis.

    Article Snippet: Cutaneous IL-22 concentrations were quantified using a mouse IL-22 enzyme-linked immunosorbent assay kit (Elabscience, catalog no. E-EL-M2446, Wuhan, China) according to the manufacturer's instructions.

    Techniques: Tissue, Cream

    Post-hoc Dunn test with Benjamini–Hochberg FDR correction for IL-22 levels.

    Journal: Frontiers in Medicine

    Article Title: Effects of topical Averrhoa bilimbi leaf extract cream on IL-22 and filaggrin in a murine model of atopic dermatitis

    doi: 10.3389/fmed.2026.1883375

    Figure Lengend Snippet: Post-hoc Dunn test with Benjamini–Hochberg FDR correction for IL-22 levels.

    Article Snippet: Cutaneous IL-22 concentrations were quantified using a mouse IL-22 enzyme-linked immunosorbent assay kit (Elabscience, catalog no. E-EL-M2446, Wuhan, China) according to the manufacturer's instructions.

    Techniques: Comparison, Positive Control, Control